Assay Instruments
A number of spectrofluorometric thermal cyclers and spectrofluorometric temperature-controlled plate readers that monitor the progress of amplification reactions (i.e., Polymerase Chain Reaction (PCR), Nucleic Acid Sequence Based Amplification (NASBA), Transcription Mediated Amplification (TMA), Rolling Circle Amplification (RCA) and Strand Displacement Amplification (SDA)) by monitoring changes in fluorescence are available and are compatible with molecular beacons. In addition, fluorospectrometers are available to monitor the fluorescence generation of molecular beacons with and without nucleic acid targets present.
Recent Publications from our group
Banada PP, Green R, Streck D, Kurathi R, Reiss R, Banik S, Montalvan I, Jones R, Marras SAE, Chakravorty S, and Alland D (2023) An expanded RT-PCR melting temperature coding assay to rapidly identify all known SARS-CoV-2 variants and sub-variants of concern. Scientific Reports 13. 21927. PMID: 38081834: PubMed Link
Ebraham L, Xu C, Wang A, Hernandez C, Siclari N, Rajah D, Walter L, Marras SAE, Tyagi S, Fine DH, Daep CA, and Chang TL (2023) Oral Epithelial cells expressing low or undetectable levels of human angiotensin-converting enzyme 2 are susceptible to SARS-CoV-2 virus infection in vitro. Pathogens 12. PMID: 37375533: PubMed Link